Canine Progesterone Levels Chart for Dog Breeders

Breeder Education

Canine Progesterone Levels Chart for Dog Breeders

Progesterone testing helps breeders and reproductive veterinarians better estimate the fertile window by tracking hormonal changes across the heat cycle. This chart is intended as an educational guide and should be used alongside serial testing and veterinary interpretation.

How to Read This Chart

In the female, progesterone begins rising before ovulation. Because values can move quickly, a single test rarely tells the full story. The most reliable timing comes from serial progesterone testing, consistent sampling, and interpretation in context with the dog’s cycle history and breeding plan.

Important: Exact timing can vary by dog, assay method, and breeding protocol. This page is educational and does not replace veterinary breeding management advice.

Key Takeaways

  • Early-cycle values are often below 1 ng/mL.
  • The LH surge often occurs around 2 ng/mL.
  • Ovulation commonly occurs around 4–10 ng/mL.
  • Canine oocytes typically require about 2–3 days after ovulation to mature.
  • Repeat testing is often needed every 24–48 hours once levels begin rising.

Typical Canine Progesterone Timing Guide

Progesterone Level Typical Interpretation What Breeders Usually Do Next
< 1.0 ng/mL Often consistent with early proestrus or baseline levels before the fertile window. Continue monitoring signs of heat and begin or continue scheduled testing as directed.
~ 2.0 ng/mL The LH surge often occurs around this point. Increase attention to timing. Serial testing becomes especially important from here forward.
4–10 ng/mL Ovulation commonly occurs in this range, though exact values can vary. Work from repeat results and your veterinarian’s protocol rather than a single number alone.
Rising after ovulation Eggs still require time to mature after ovulation before they are ready for fertilization. Confirm timing with follow-up testing and your breeding method plan.
Serial trend matters most A progression of values is more useful than one isolated result. Use repeat testing, veterinary interpretation, and semen type logistics to finalize timing.
This chart is intentionally conservative and educational. It avoids assay-specific promises and should not be used as a substitute for veterinary timing advice.
Progesterone results should always be interpreted in the context of serial testing and veterinary guidance. Individual dogs may vary, and breeding protocols may differ depending on semen type and reproductive management.

Why Serial Testing Matters

Progesterone can rise quickly once the fertile window approaches. Because canine reproduction timing depends on both hormonal progression and egg maturation, breeders usually rely on a series of tests rather than one isolated value.

  1. Start testing after the onset of heat based on your veterinarian’s recommendation.
  2. Track the trend as progesterone begins to rise.
  3. Retest more frequently once values approach the ovulatory window.
  4. Use the full trend to plan breeding timing with greater confidence.

Helpful Breeder Reminder

Breeding dates alone are often poor predictors because the canine egg is not ready to be fertilized immediately at ovulation. That is one reason progesterone timing is so valuable for both natural breedings and assisted reproduction planning.

If you offer educational content on your store, this page works best when paired with a clear “contact your reproductive veterinarian” disclaimer.

Mini VIDAS Progesterone Testing Supplies

Looking for equipment and supplies for canine progesterone testing? Explore the refurbished Mini VIDAS machine and Mini VIDAS progesterone test kits available in our store.